Carnitine transport and fatty acid oxidation ScienceDirect Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Carnitine Metabolism and Its Role in Fatty Acid Oxidation Creative Proteomics Activation and Transportation of Fatty Acids to the Mitochondria via the Carnitine Shuttle with Animation Animations PharmaXChange.info